Dr.Pourhossein | MUI Profession Profile

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	Meraj Pourhossein , PhD Associate Professor Google Scholar Research Gate Orcid Isid Linkedin Scopus Phone: +989100017849 Email: dr.meraj.p@gmail.com Date of birth : 00 CV: CV of Dr.Pourhossein.pdf (94.24 KB) Personal information Research Teaching School : School of Nutrition and Food Sciences Department : Department of Food Sciences and Technology Research Center : Food Security Research Center work address : School of Nutrition and Food Science, Isfahan University of Medical Sciences (MUI), Hezarjerib Ave, Isfahan, Iran, Postal Code: 81746-73461 Education : EMPLOYMENT: 1999-2003 PhD. Molecular Biology & Biotechnology, Univ. of Manchester (UK) 1998-1999 MSc. Molecular Biology, Univ. of Manchester (UK) 1989-1992 MSc. Microbiology, University of Tehran (Iran) 1985-1989 BSc. Microbiology, University of Isfahan (Iran) AND PROFESSIONAL INTERESTS: I am interested in Molecular biology related techniques. I have experience in the following techniques: In silico analysis, homology studies, Electron Microscopy (SEM and TEM), Atomic Force Microscopy (AFM), DNA purification from Animal and bacterial cells, preparation of plasmid DNA , PCR based techniques, Using Restriction Endonucleases for Partial & Multiple Digestions of DNA, ligation and cloning techniques, transformation of plasmid DNA into cells, screening of recombinant DNA, DNA sequencing, Western blotting, Coomassie blue and Silver staining, complementation assays, protein purification, production of polyclonal antibodies, large scale protein production for crystallographic analysis, site directed mutagenesis, hybridization studies, phage sensitivity analysis, radioactive incorporation and autoradiography. Relevant Work Experience : EMPLOYMENT: International affairs officer of School of Nutrition & Food Sciences.2019-2020 Lecturer in Molecular Biology, Molecular Nutrition & Food science related courses, Department of Nutritional Sciences, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences. 2013- 2019 Lecturer in Molecular Biology & Biotechnology, Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran. 2010- 2013 Visiting Scientist, Faculty of life sciences, Manchester University. 2008- 2010 Lecturer in Molecular Biology & Biotechnology, Dep. of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Iran. 2004- 2008 Project assistant in the University of Manchester. 2003-2004 Demonstrator in MCC for IT skills in the Univ. of Manchester. 2000-2003 Lecturer in Microbiology and Electron Microscopy, School of Medicine, Dept. of Anatomy, Isfahan, Iran.1992-1998 Professional Memberships & Qualification : Extra Curricular Activities/ Interest(personal url) : Grants & Awards : Patents : Presentations & Poster Sessions : Steroid Dependent and Independent Ocular Findings in Iranian Children with Nephrotic Syndrome. Method of the Year 2010, Strategy, application and its future directions towards clinical application. Overproduction of clavulanic acid by UV mutagenesis of Streptomyces clavuligerus. Antibacterial effect of Authochlorous Lactobacillus strains isolated from traditional yogurts. Optimisation of Candida rugosa Lipase Esterase Activity. Gene finding using HMM in prokaryotic genomes. Inhibitory effect of Pseudomonas aeroginosa isolated from oral cavity on Methicilin Resistant Staphylococcus Aureus (MERSA) strains. Research Experience : Rouigari, M., Dehbashi, M., Ghaedi, K., Pourhossein, M. Targetome analysis revealed involvement of MIR-126 in neurotrophin signaling pathway: A possible role in prevention of glioma development. Cell Journal. 20(2):150-156 (2018) Korbekandi H, Mohseni S, Mardani Jouneghani R, Pourhossein M, Iravani S. Biosynthesis of silver nanoparticles using Saccharomyces cerevisiae. Artif Cells Nanomed Biotechnol. 2016;44(1):235-9. Sehhati MR., Mehri AR., Rabbani H., Pourhossein M., Stable Gene Signature Selection for Prediction of Breast Cancer Recurrence Using Joint Mutual Information. IEEE/ACM Trans. Comput. Biology Bioinform. 12(6): 440-1448 (2015) Korbekandi H., Jouneghani RM., Mohseni S., Pourhossein M., Iravani S., Synthesis of silver nanoparticles using biotransformations by Saccharomyces boulardii. GREEN PROCESSING AND SYNTHESIS. Volume: 3, Issue: 4 Pages: 271-277 (2014) Ghavimi R, Pourhossein M, Ghaedi K, Alesahebfosoul F, Honardoost MA, Maracy MR. Genetic association of rs1520333 G/A polymorphism in the IL7 gene with multiple sclerosis susceptibility in Isfahan population. Adv Biomed Res. 2014 Nov 29;3:238. Pourhossein M, Ghavimi R, Alsahebfosoul F, Ghaedi K. Association between rs1800795 (-174 G/C) Polymorphism in the Promoter of IL6 Gene and Risk of Relapsing-Remitting Multiple Sclerosis (RRMS) in Isfahan Population. Open Journal of Genetics, Vol.4 No.5, 407-413. Ghavimi, R., Pourhossein, M., Limitations of clinical application of siRNA delivery based on non-viral vectors (Review). Journal of Isfahan Medical School, Volume 32, Issue 272, 2014, Pages 34-48 Pourhossein M, Korbekandi H. Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli. Adv Biomed Res 2014;3:82. Kheradmand E, Pourhossein M, Amini G, Saadatnia M. Factor V Leiden does not have a role in cryptogenic ischemic stroke among Iranian young adults. Advanced Biomedical Research, 2014;3: 80 Tajadini, Mohammad Hassan; Khadem, Houra; Pourhosein, Meraj; Sabzghabaee, Ali Mohammad; Hemati, Simin; Sadeghi, Hamid Mirmohammad. Investigating the Prevalence of BRCA1 and BRCA2 Gene Mutations in Patients with Breast Cancer. J Isfahan Med Sci 2013; 30 (218): 2217-2224. Research Interests : K5 heparan lyase combines features of viral tail spike hydrolases and bacterial polysaccharide lyases.(2010) Expression of E.coli capsular polysaccharide requires the KfiB protein:A Structural based analysis.(2008) Identification that KfiA , a protein Essential for the Biosynthesis of the E.coli K5 capsular polysaccharide, is an α–UDP-GlcNAc Glycosyltransferase.(2000) Teaching Experience : Teaching Interests :

Provider

Meraj Pourhossein , PhD

Associate Professor

Phone: +989100017849
Email: dr.meraj.p@gmail.com
Date of birth : 00
CV: CV of Dr.Pourhossein.pdf (94.24 KB)

School :

School of Nutrition and Food Sciences

Department :

Department of Food Sciences and Technology

Research Center :

Food Security Research Center

work address :

School of Nutrition and Food Science, Isfahan University of Medical Sciences (MUI), Hezarjerib Ave, Isfahan, Iran, Postal Code: 81746-73461

Education :

EMPLOYMENT: 1999-2003 PhD. Molecular Biology & Biotechnology, Univ. of Manchester (UK) 1998-1999 MSc. Molecular Biology, Univ. of Manchester (UK) 1989-1992 MSc. Microbiology, University of Tehran (Iran) 1985-1989 BSc. Microbiology, University of Isfahan (Iran) AND PROFESSIONAL INTERESTS: I am interested in Molecular biology related techniques. I have experience in the following techniques: In silico analysis, homology studies, Electron Microscopy (SEM and TEM), Atomic Force Microscopy (AFM), DNA purification from Animal and bacterial cells, preparation of plasmid DNA , PCR based techniques, Using Restriction Endonucleases for Partial & Multiple Digestions of DNA, ligation and cloning techniques, transformation of plasmid DNA into cells, screening of recombinant DNA, DNA sequencing, Western blotting, Coomassie blue and Silver staining, complementation assays, protein purification, production of polyclonal antibodies, large scale protein production for crystallographic analysis, site directed mutagenesis, hybridization studies, phage sensitivity analysis, radioactive incorporation and autoradiography.

Relevant Work Experience :

EMPLOYMENT: International affairs officer of School of Nutrition & Food Sciences.2019-2020 Lecturer in Molecular Biology, Molecular Nutrition & Food science related courses, Department of Nutritional Sciences, School of Nutrition and Food Sciences, Isfahan University of Medical Sciences. 2013- 2019 Lecturer in Molecular Biology & Biotechnology, Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran. 2010- 2013 Visiting Scientist, Faculty of life sciences, Manchester University. 2008- 2010 Lecturer in Molecular Biology & Biotechnology, Dep. of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Iran. 2004- 2008 Project assistant in the University of Manchester. 2003-2004 Demonstrator in MCC for IT skills in the Univ. of Manchester. 2000-2003 Lecturer in Microbiology and Electron Microscopy, School of Medicine, Dept. of Anatomy, Isfahan, Iran.1992-1998

Professional Memberships & Qualification :

Extra Curricular Activities/ Interest(personal url) :

Grants & Awards :

Patents :

Presentations & Poster Sessions :

Steroid Dependent and Independent Ocular Findings in Iranian Children with Nephrotic Syndrome. Method of the Year 2010, Strategy, application and its future directions towards clinical application. Overproduction of clavulanic acid by UV mutagenesis of Streptomyces clavuligerus. Antibacterial effect of Authochlorous Lactobacillus strains isolated from traditional yogurts. Optimisation of Candida rugosa Lipase Esterase Activity. Gene finding using HMM in prokaryotic genomes. Inhibitory effect of Pseudomonas aeroginosa isolated from oral cavity on Methicilin Resistant Staphylococcus Aureus (MERSA) strains.

Research Experience :

Rouigari, M., Dehbashi, M., Ghaedi, K., Pourhossein, M. Targetome analysis revealed involvement of MIR-126 in neurotrophin signaling pathway: A possible role in prevention of glioma development. Cell Journal. 20(2):150-156 (2018) Korbekandi H, Mohseni S, Mardani Jouneghani R, Pourhossein M, Iravani S. Biosynthesis of silver nanoparticles using Saccharomyces cerevisiae. Artif Cells Nanomed Biotechnol. 2016;44(1):235-9. Sehhati MR., Mehri AR., Rabbani H., Pourhossein M., Stable Gene Signature Selection for Prediction of Breast Cancer Recurrence Using Joint Mutual Information. IEEE/ACM Trans. Comput. Biology Bioinform. 12(6): 440-1448 (2015) Korbekandi H., Jouneghani RM., Mohseni S., Pourhossein M., Iravani S., Synthesis of silver nanoparticles using biotransformations by Saccharomyces boulardii. GREEN PROCESSING AND SYNTHESIS. Volume: 3, Issue: 4 Pages: 271-277 (2014) Ghavimi R, Pourhossein M, Ghaedi K, Alesahebfosoul F, Honardoost MA, Maracy MR. Genetic association of rs1520333 G/A polymorphism in the IL7 gene with multiple sclerosis susceptibility in Isfahan population. Adv Biomed Res. 2014 Nov 29;3:238. Pourhossein M, Ghavimi R, Alsahebfosoul F, Ghaedi K. Association between rs1800795 (-174 G/C) Polymorphism in the Promoter of IL6 Gene and Risk of Relapsing-Remitting Multiple Sclerosis (RRMS) in Isfahan Population. Open Journal of Genetics, Vol.4 No.5, 407-413. Ghavimi, R., Pourhossein, M., Limitations of clinical application of siRNA delivery based on non-viral vectors (Review). Journal of Isfahan Medical School, Volume 32, Issue 272, 2014, Pages 34-48 Pourhossein M, Korbekandi H. Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli. Adv Biomed Res 2014;3:82. Kheradmand E, Pourhossein M, Amini G, Saadatnia M. Factor V Leiden does not have a role in cryptogenic ischemic stroke among Iranian young adults. Advanced Biomedical Research, 2014;3: 80 Tajadini, Mohammad Hassan; Khadem, Houra; Pourhosein, Meraj; Sabzghabaee, Ali Mohammad; Hemati, Simin; Sadeghi, Hamid Mirmohammad. Investigating the Prevalence of BRCA1 and BRCA2 Gene Mutations in Patients with Breast Cancer. J Isfahan Med Sci 2013; 30 (218): 2217-2224.

Research Interests :

K5 heparan lyase combines features of viral tail spike hydrolases and bacterial polysaccharide lyases.(2010) Expression of E.coli capsular polysaccharide requires the KfiB protein:A Structural based analysis.(2008) Identification that KfiA , a protein Essential for the Biosynthesis of the E.coli K5 capsular polysaccharide, is an α–UDP-GlcNAc Glycosyltransferase.(2000)